Welcome to the official website of the laboratory of Maria Ninova, Assistant Professor at the Department of Biochemistry, University of California, Riverside. The lab opened on July 1st 2021 and is actively recruiting curiosity-driven and motivated team members interested in epigenetic regulation, small non-coding RNAs, and transposon control in germ cells and animal development at different career levels, including technicians, undergraduate, graduate, and postdoctoral researchers - click here for more details!
An organism’s genome contains all the information necessary for its development and function, including genes that encode RNA and proteins, regulatory regions that coordinate gene activity to form different cells and respond to stimuli, structural regions responsible for the stability, organization and faithful DNA transmission during cell division, as well as “parasitic” elements such as jumping genes (transposons) and regions of no or unknown role. It is essential that this information is organized and switched on and off in a controlled manner, so that only pieces relevant to a specific cell type and function are active at a given time, while irrelevant or harmful genetic elements are silenced. One of the most important means of genome regulation in eukaryotes is through the association of DNA with histone proteins into a higher order chromatin structure. Post-translational histone modifications can alter chromatin density and recruit diverse downstream regulatory and structural factors that modulate gene activity. Our lab is interested in fundamental mechanisms that govern the formation of silencing chromatin marks at different genomic regions. We address these questions by a combiation of molecular biology, imaging, genomics (RNA-seq, ChIP-seq), and proteomics approaches. Most of our projects utilize the Drosophila melanogaster model system, which laid the foundations of modern genetics and has been at the frontier of chromatin, piRNA (see below), and transposon research. Areas of specific interest include (but are not limited to):
piRNAs are a class of short non-coding RNAs produced in the germline and some somatic tissues of animals that associate with Argonaute proteins from the Piwi clade, and function to recognize and silence transposons that can otherwise damage DNA. Defects in the piRNA pathways lead to transposon activation, defects in gametogenesis, and sterility. Remarkably, specific piRNA-loaded Piwi-s have the ability to enter the nucleus and induce transcriptional silencing of transposons by recruiting repressive chromatin-modifying factors - a great example of non-coding RNAs triggering epigenetic modifications in animal systems. Recent studies identified several new factors essential for Piwi function. For example, it recently emerged that
the SUMO (Small Ubiquitin-Like Modifier) pathway is required for transposon silencing and repessive chromatin formation at piRNA targets. However, the precise mechanism by which piRNA/Piwi complexes recruit the silencing effectors is not well understood. Our lab aims to use a combination of molecular biology, genomics, and proteomics strategies, to dissect the process of piRNA-mediated silencing.
Heterochromatin was historically identified as the dark staining portion of interphase chromatin which remains condensed and is typically (but not always) transcriptionally silenced. Heterochromatin is essnetial for many aspects of cell function and development, such as dosage compensation, lineage-specific gene silencing during differentiation, maintaining the integrity of the repetitive chromosome ends, and repression of transposons. Histone 3 lysine 9 trimethylation (H3K9me3) is a highly conserved hallmark of silencing and heterochromatin that occupies a substantial portion of the eukaryotic genomes and is implicated in the compaction of repeat-rich sequences near centromeres and telomeres, and transposon repreression. In addition, this mark is found on dozens of host genes and regulatory regions outside constitutive heterochromatin, and appears to play a role in
gene regulation and cell specification. Our lab is interested in gaining a deeper understanding of the biological role of H3K9me3 in different cellular and genomic contexts.
The vast majority of histone and DNA modifications are erased during gametogenesis and fertilization, and need to be re-established de novo in a cell-specific manner during the development of the new organism. We are interested in the factors and molecular mechanisms that orchestrate the initiation and maintenance of heterochromatin at host genes and transposons from the zygote throughout embryogenesis using the fruit fly embryo system as a paradigm.
All publications: Google Scholar
We are looking for motivated individuals at different career levels interested in the lab’s scientific areas to join my research team. Available projects employ a variety of classic and state-of-the-art approaches, and provide the opportunity to obtain training in either or both experimental biology and bioinformatics. PhD and postdoc positions are open to both US and international (subject to U.S. visa rules) applicants. Women and underrepresented minorities are encouraged to apply!
Applicants should be near completion or have recently completed a PhD program in the following or related disciplines: molecular biology, biochemistry, biophysics, genetics, bioinformatics, and have either substantial hands-on experience with standard biochemistry and molecular biology techniques (such as PCR, Western blotting, protein purification, cloning, etc.), and cell culture, or experience in computational analysis of sequencing data and comparative genomics. Experience with Drosophila is a plus, but is not required. Specific projects will be tailored to the applicant experience and interests - contact Maria directly to discuss current research opportunities. Please include a short paragraph describing your scientific interests and motivations, resume/CV, contacts of three referees, and if available - an example publication (pre-print is fine) reflecting prior work.
Applicants must be admitted to one a graduate program at UC Riverside, preferably:
Information regarding eligibility criteria, application deadlines (usually in the fall), and application process is available on each program’s website. All programs require students to complete 5-10 weeks laboratory rotations in the first year, before further selection for doctoral thesis advisor/lab is made. Prospective students interested in rotating in the lab are encouraged to get in touch with Maria in advance to discuss research opportunities. Please include a short paragraph describing your prior research experience (if any), scientific interests and motivations, resume/CV, undergraduate transcript, and if available - an example publication (pre-print is fine) reflecting prior work. Many of the projects will involve next generation sequencing (e.g. RNA-seq, ChIP-seq), and are particularly suitable for individuals who wish to obtain a “mixed” training in biology and bioinformatics. It is not necessary to have prior programming experience, however, background in a biology-related discipline is helpful. Co-mentoring arrangements jointly with other PIs are also a possibility.
We welcome applications for junior staff positions (laboratory assistant or junior specialist) from individuals interested in taking part in our research. Candidates should have at least a bachelor’s degree or equivalent in life sciences and have some prior lab experience. These positions are ideal for recent graduates seeking to gain extra research experience. For inquiries, contact Maria and include [TECHNICIAN] (with the brackets and upper case letters) in the subject line. Please include a short paragraph describing your prior research experience, scientific interests and motivations, resume/CV, undergraduate transcript, and if available - an example publication (pre-print is fine) reflecting prior work.
Opportunities are available for undergraduate students with strong commitment to research. Students interested in the lab areas of study should contact Maria to discuss potential projects and timelines. Please include [UNDERGRAD] (with brackets and upper case) in the subject line, briefly describe your research interests and goals (see tips for contacting faculty), relevant courses, and attach a copy of your transcript.
To get in touch with Maria, please send an email to:
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